Principal Investigator
Volker Brözel
"I started out in food science but soon found bacteria more interesting. After completing B.Sc. in Food Science in the beautiful Stellenbosch, South Africa, I pursued M.Sc. and Ph.D. at the University of Pretoria. I liked being a student so much that I never left university, teaching and mentoring students while studying how bacteria behave outside the petri dish – ecophysiology. My work at the University of the Western Cape (1994 – 1997) and later back at the University of Pretoria (1998 – 2003) focused on bacterial biofilms in industrial and drinking water systems. I moved to South Dakota State University in the USA in 2003. Living in the agricultural heartland of the USA re-directed my focus to the bacteria in soil."
Current Graduate Students
Armaan Kaur Sandhu
Ph.D. Candidate
"A part of my doctoral research centers on the investigation of the phenotypic and root attachment related properties of Bradyrhizobium diazoefficiense in presence of their natural nutrional environment i.e root exudate compounds. Additionally, I am delving into a rarely known bacterial phenomenon: Sibling Rivalry. We have observed the emergence of demarcation lines within swimming colonies of Bradyrhizobium, further directing my investigation into the underlying physiological conditions and causal factors responsible for this intriguing occurrence."
Research Interest: Beneficial plant-microbe interaction, Soil Microbiology, Fermentation microbiology
Hobbies: Painting, Gardening, Travelling
Here is where you can know me more:
LinkedIn : https://www.linkedin.com/in/armaan-kaur-sandhu-454062184/
Google Scholar: https://scholar.google.com/citations?user=JtWHUxcAAAAJ&hl=en&oi=ao
Sukhvir Kaur Sarao
Ph.D. student
"I am a PhD student in the Brozel lab. I work with a soil bacterium Bradyrhizobium, trying to unfurl the various phenotypic heterogenous populations within the so-called homogenous pure culture. I am also working to isolate novel Bradyrhizobium strains from various soil types from South Dakota."
Outside lab, I love to take a nap ‘anytime’ ‘anywhere’.
Muhammad Yasir Afzal
Ph.D. student
"I came from Pakistan, and I did my MS in Microbiology from the Government College University Faisalabad (GCUF).
I have interest in exploring the bacterial world, especially in soil.
I am currently working on:
- Response of Bacillus cereus when encountered by growing roots.
- Why Bacillus populations in soil do not increase in numbers despite being fast-growing bacteria."
Johnathan Orosz
Ph.D. student
I am currently trying to figure out how to encapsulate known diazotrophs and phosphate-solubilizers, i.e. plant growth promoting bacteria (PGPB), in biopolymers, so that we can do it with novel bacterial consortia that will thrive in real field conditions. The purpose of this is to give farmers a way to grow crops using less synthetic nitrogen and phosphate inputs, so they can save money and keep intensively-used soil healthy. Biofertilizers, or inoculants, have been used for some time. However, the puzzle remains: How do we formulate PGPB biofertilizers that are stable and perform consistently over time?
In my free time I go for long walks with my dog Zhuri and reflect on nature.
Undergraduate students
JaLeigha Kambeitz
Spring 2024
Topic of Research: Bacterial antagonists of Bacillus in soil
Recent Graduate Students
Dr. Bikram Das
Ph.D. (2022)
Thesis : Microbial Communities and Nitrogen Dynamics in Prairie and Cropland Soils
LinkedIn: https://www.linkedin.com/in/bikram-kumar-d-5b4a05167/
Dr. Amrit Koirala
Ph.D. (2021)
LinkedIn: https://www.linkedin.com/in/amrit-koirala-41037392/
Amina Baniya
M.S (2020)
Thesis: Interaction of Streptomyces Closely Associated with Bacillus in Nitrogen-limiting Conditions
LinkedIn: https://www.linkedin.com/in/amina-baniya-ab2278101/?originalSubdomain=np
Dr. Gitanjali NandaKafle
Ph.D. (2018)
Thesis: The Role of Genomic Versatility in Multi-Niche Preferences of Escherichia coli
LinkedIn: https://www.linkedin.com/in/gitanjali-nandakafle-2019g/
Previous Graduate Students
- Nabilah Alshibli
MS (2018)
- Dr. Laura J. White
Ph.D. (2017)
- Tyrel Deutscher
MS (2016)
- Ryan Schmid
-
MS (2014)
-
Ph.D. (2011)
- Ursula Waack
MS (2011)
- Kelly Lehnert
-
MS (2008)
- Amy Christie
MS (2006)
- Yun Luo
MS (2006)
Recent Undergraduate Students
Kallie Crowl
Fall 2022, Summer 2023
Topic of Research: Lifestyle of Bradyrhizobium Diazoefficiens USDA 110: Planktonic or Biofilm
"In the fall of 2022, in identified the adherence preference of Bradyrhizobium diazoefficiens USDA 110. We wanted to know if it is a natural biofilm former or likes to stay in planktonic phase. In summer 2023 I assisted Armaan in studying sibling rivalry in USDA 110 and nodule occupancy of USDA 110 versus other strains"
Cole Adam
Fall 2021, Spring 2022
Topic of research: Bacterial genetics.
"My research targeted alternative nitrogen-fixing pathways in Bacillus able to grow without combined nitrogen."
McKenzie Brown
Spring 2022, Fall 2022
Topic of Research: Soy Root Adherence and Adherence Related Properties of Bradyrhizobium japonicum USDA 20 are Altered in Response to Diverse Root Exudates and Soil Extract
"The purpose of my research was to analyze the effect of various soybean root exudates on root attachment related surface and phenotypic properties of Bradyrhizobium japonicum USDA 20."
Mitchal Eichacker
Spring 2023
"I isolated Bradyrhizobium from local soybean field soils using selective media, working under supervision of Sukhvir Kaur. "
Emma Osmundson
Fall 2022
Topic of research: Bradyrhizobium
"We isolated Bradyrhizobium from soil samples using selective media, and characterized them down to species level."
Amelia (Mia) Thoennes
Fall 2021-Summer 2022
Topic of research: Identifying Pigmented Microbes found in Soil
"I took soil samples from Watertown, SDSU Campus, and parts of Colorado, and diluted and plated these to obtain pigmented bacteria. I would then wait for pigmented microbes to grow in hopes of identifying the microbe, as well as using the microbe to create art with."
Laney Brown
Spring/Summer 2021
Topic of research: Escherichia coli & Nitrogen-fixing bacteria
" I worked with Dr. Brozel for my capstone project to determine the E. coli phylogroups within the Brookings community. I was then hired as his laboratory assistant for an additional three months where we isolated Bacillus able to grow without combined nitrogen, and characterized these."
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